Standard Doxosome™ Kit-Doxorubicin Liposomes (PEGylated)
SKU# DOX-3000
Description
The kit is composed of one vial of PEGylated doxorubicin liposomes and one vial of PEGylated control liposomes. PEGylated doxorubicin liposome formulation is similar to commercial Doxil® in size, lipid composition and the amount of encapsulated drug. The formulation is for research purposes only and for injection to laboratory animals. Doxorubicin drug is already loaded into the liposomes using remote loading by ammonium sulfate gradient. The formulation is ready for injection. The control formulation of PEGylated doxorubicin is similar to PEGylated doxorubicin in size and lipid composition, but it does not contain doxorubicin drug. The formulation is made in ammonium sulfate gradient.
Surface reactive liposome formulations suitable for conjugation to various antibodies, proteins, peptides and ligands are also available. For more information about surface reactive liposomes (Immunsome®) see here.
For more information about surface reactive doxorubicin liposomes (Immundox®) suitable for conjugation see here.
Formulation Information
Standard Doxosome™ Kit-Doxorubicin Liposomes (PEGylated)
| 2-Vial Kit for Standard Doxosome™ (PEGylated) | Specification |
|---|---|
| Vial 1 | Preformed liposomes composed of HSPC:Cholesterol:DSPE-PEG(2000) (57:38:5 molar ratio) |
| Vial 2 | Preformed liposomes composed of HSPC:Cholesterol:DSPE-PEG(2000) (57:38:5 molar ratio) |
| Lipid Composition for Vial 1 | Concentration (mg/ml) | Concentration (mM) | Molar Ratio Percentage |
|---|---|---|---|
| Hydrogenated Soy PC | 9.58 | 12.22 | 57 |
| Cholesterol | 3.19 | 8.26 | 38 |
| DSPE-PEG(2000) | 3.19 | 1.14 | 5 |
| Total | 15.96 mg/ml | 21.62 mM | 100 |
| Buffers, Liposome Size and Encapsulated Drug Concentration for Vial 1 | Specification |
|---|---|
| Inside Buffer | Ammonium Sulfate |
| Outside Buffer | Histidine/Sucrose Buffer |
| pH | 6.5 |
| Liposome Size | 80-100 nm |
| Encapsulated Doxorubicin | 2 mg/ml (3.45 mM) |
| Lipid Composition for Vial 2 | Concentration (mg/ml) | Concentration (mM) | Molar Ratio Percentage |
|---|---|---|---|
| Hydrogenated Soy PC | 9.58 | 12.22 | 57 |
| Cholesterol | 3.19 | 1.14 | 38 |
| DSPE-PEG(2000) | 3.19 | 8.26 | 5 |
| Total | 15.96 mg/ml | 21.62 mM | 100 |
| Buffers and Liposome Size for Vial 2 | Specification |
|---|---|
| Inside Buffer | Ammonium Sulfate |
| Outside Buffer | Histidine/Sucrose Buffer |
| pH | 6.5 |
| Liposome Size | 80-100 nm |
Liposome Particle Calculator
Doxorubicin liposomes are unilamellar and sized to 100 nm. The molar concentration of liposome is 21.62 mM. By having liposome diameter (nm) and lipid concentration (µM), you can calculate the total number of the lipids in one liposome and the number of the liposomes in one milliliter of the liposome solution. To use the calculator click here.
Appearance
Doxosome™ is a red, and Doxosome™ Control is a white translucent liquid both made of nano size unilamellar liposomes. Usually, due to the small size of liposomes, no settling will occur in the bottom of the vial. The liposomes are packaged in amber vials.
Educational Video
Ordering/Shipping Information
- All liposome based formulations are shipped on blue ice at 4°C in insulated packages using overnight shipping or international express shipping.
- Liposomes should NEVER be frozen. Ice crystals that form in the lipid membrane can rupture the membrane, change the size of the liposomes and cause the encapsulated drug to leak out. Liposomes in liquid form should always be kept in the refrigerator.
- Clients who order from outside of the United States of America are responsible for their government import taxes and customs paperwork. Encapsula NanoSciences is NOT responsible for importation fees to countries outside of the United States of America.
- We strongly encourage the clients in Japan, Korea, Taiwan and China to order via a distributor. Tough customs clearance regulations in these countries will cause delay in custom clearance of these perishable formulations if ordered directly through us. Distributors can easily clear the packages from customs. To see the list of the distributors click here.
- Clients ordering from universities and research institutes in Australia should keep in mind that the liposome formulations are made from synthetic material and the formulations do not require a “permit to import quarantine material”. Liposomes are NOT biological products.
- If you would like your institute’s FedEx or DHL account to be charged for shipping, then please provide the account number at the time of ordering.
- Encapsula NanoSciences has no control over delays due to inclement weather or customs clearance delays. You will receive a FedEx or DHL tracking number once your order is confirmed. Contact FedEx or DHL in advance and make sure that the paperwork for customs is done on time. All subsequent shipping inquiries should be directed to Federal Express or DHL.
Storage and Shelf Life
Storage
Doxosome™ products should always be stored at in the dark at 4°C, except when brought to room temperature for brief periods prior to animal dosing. DO NOT FREEZE. If the suspension is frozen, the encapsulated drug can be released from the liposomes thus limiting its effectiveness. In addition, the size of the liposomes will also change upon freezing and thawing.
Shelf Life
Doxosome™ is made on daily basis. The batch that is shipped is manufactured on the same day. It is advised to use the products within 4 months of the manufacturing date.
References and background reading
1. Hermanson GT. Bioconjugate techniques. Academic press; 2013 Jul 25.
