PS-based Fluoroliposome®-Azide for Tracking and Detection
Technical Information
PS-based Fluoroliposome®-Azide for Tracking and Detection
| Lipid Composition | Concentration (mg/ml) | Concentration (mM) | Molar Ratio Percentage |
|---|---|---|---|
| Total | mg/ml | mM | 100 |
| L-α-phosphatidylserine | 20 | ||
| L-α-phosphatidylcholine | 49 | ||
| Cholesterol | 30 | ||
| 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(6-azidohexanoyl) (ammonium salt) | 1 |
| Fluorescent Dye | Excitation/Emission (nm) | Molecular Structure |
|---|---|---|
| 1,1'-Dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI) | 549/565 |  |
| 3,3'-Dilinoleyloxacarbocyanine perchlorate (DiO) | 484/501 |  |
| 1,1'-Dioctadecyl-3,3,3',3'-tetramethylindodicarbocyanine, 4-chlorobenzenesulfonate salt (DiD) | 644/665 |  |
| 1,1'-Dioctadecyl-3,3,3',3'-tetramethylindotricarbocyanine iodide (DiR) | 750/780 |  |
| 4-(4-(Dihexadecylamino)styryl)-N-methylpyridinium iodide (DiA) | 456/590 |  |
| 1,2-Distearoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (ammonium salt) (NBD on head group) | 460/535 |  |
| 1-Palmitoyl-2-{12-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]dodecanoyl}-sn-glycero-3-phosphocholine (NBD on fatty acid tail) | 460/534 |  |
| 1,2-Dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (ammonium salt) (Rhodamine lipid) | 560/583 |  |
| 1,2-Dioleoyl-sn-glycero-3-phosphoethanolamine-N-(5-dimethylamino-1-naphthalenesulfonyl) (ammonium salt) (Dansyl lipid) | 336/513 |  |
| 1,2-Dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(1-pyrenesulfonyl) (ammonium salt) (Pyrene lipid) | 351/379 |  |
| Buffer and Liposome Size | Specification |
|---|---|
| Buffer | Phosphate Buffered Saline* |
| pH | 7.4 |
| Liposome Size | 100 nm |
| * If you prefer different buffers, please specify in your order. | |
Technical Notes
- If you are planning to conjugate a molecule to the surface of the liposomes then keep in mind that PS-based liposomes are highly negative in charge. It is better to use a reactive dye or molecule that is more neutral in charge rather than a dye or molecule that has a strong positive charge which can cause aggregation.
- Liposomes should be kept at 4 °C and NEVER be frozen.
Appearance
PS-based Fluoroliposome®-Azide formulation is a colored translucent liquid, and the color depends on the type of the fluorescent dye that is used. Usually due to the small size of liposomes no settling will occur in the bottom of the vial. The liposomes are packaged in an amber vial.
Ordering/Shipping Information
- All liposome based formulations are shipped on blue ice at 4 °C in insulated packages using overnight shipping or international express shipping.
- Liposomes should NEVER be frozen. Crystal ice that is formed in the lipid membrane can rupture the membrane, change the size of the liposomes and cause the encapsulated drug to leak out. Liposomes in liquid form should always be kept in the refrigerator.
- Clients who order from outside of the United States of America are responsible for their government import taxes and customs paperwork. Encapsula NanoSciences is NOT responsible for importation fees to countries outside of the United States of America.
- We strongly encourage the clients in Japan, Korea, Taiwan and China to order via a distributor. Tough customs clearance regulations in these countries will cause delay in custom clearance of these perishable formulations if ordered directly through us. Distributors can easily clear the packages from customs. To see the list of the distributors click here.
- Clients ordering from universities and research institutes in Australia should keep in mind that the liposome formulations are made from synthetic material and the formulations do not require a “permit to import quarantine material”. Liposomes are NOT biological products.
- If you would like your institute’s FedEx or DHL account to be charged for shipping then please provide the account number at the time of ordering.
- Encapsula NanoSciences has no control over delays due to inclement weather or customs clearance delays. You will receive a FedEx or DHL tracking number once your order is confirmed. Contact FedEx or DHL in advance and make sure that the paperwork for customs is done on time. All subsequent shipping inquiries should be directed to Federal Express or DHL.
Storage and Shelf Life
Storage
PS-based Fluoroliposome®-Azide should always be stored at in the dark at 4 °C, except when brought to room temperature for brief periods prior to animal dosing. DO NOT FREEZE. ENS is not responsible for results generated by frozen product.
Shelf Life
Fluoroliposome® products are made on daily basis. The batch that is shipped is manufactured on the same day. It is advised to use the products within 60 days of the manufacturing date.
