PG-based Fluoroliposome®-DBCO for Tracking and Detection

Size Cat no Fluorescent Moiety Price Quantity Subtotal
2-ml FPG-322 None $850.00
$0.00
2-ml FPG-323 DiA $950.00
$0.00
2-ml FPG-324 DiD $950.00
$0.00
2-ml FPG-325 DiI $950.00
$0.00
2-ml FPG-326 DiO $950.00
$0.00
2-ml FPG-327 DiR $950.00
$0.00
2-ml FPG-328 Dansyl lipid (headgroup) $950.00
$0.00
2-ml FPG-329 NBD lipid (fatty acid tail) $950.00
$0.00
2-ml FPG-330 NBD lipid (headgroup) $950.00
$0.00
2-ml FPG-331 Pyrene lipid (headgroup) $950.00
$0.00
2-ml FPG-332 Rhodamine lipid (headgroup) $950.00
$0.00
Bulk & Custom sizes Checkout
Cart Total $0.00

Description

Fluoroliposome® containing a surface reactive lipid can be used in various ways and in different types of experiments.

Phosphatidylglycerol (PG) based liposomes are negatively charge liposomes.

If you are planning to use a specific fluorophore with a certain property and excitation and emission range, you can simply conjugate the dye (provided that it is already tagged with DBCO or azide) to the surface reactive liposome containing azide or DBCO using click chemistry.

There is a very large catalog of azide reactive dyes that are commercially available by various chemical vendors such as click chemistry tools, Lumiprobe (azide dyes) and many more. In most cases you need to use a reactive liposome formulation that does not contain any fluorophores (eg. FPG-331) and do the conjugation by following few easy steps.

The surface reactive liposomes are also available with various types of fluorophores such as lipophilic fluorophores incorporated into the lipid membrane of the liposomes with different excitation and emission ranges such as DiA (ex/em: 456/590 nm) , DiD (ex/em: 644/665 nm), DiI (ex/em: 549/565 nm),  DiO (ex/em 484/501 nm), DiR (ex/em: 750/780 nm), NBD headgroup tagged lipid (ex/em: 460/535 nm), NBD fatty acid tail tagged lipid (ex/em: 460/534), rhodamine headgroup tagged lipid (ex/em: 560/583 nm) , dansyl headgroup tagged lipid (ex/em: 336/513 nm) and pyrene headgroup tagged lipid (ex/em: 351/379 nm). These liposomes are suitable for conjugation of a non-fluorescent moiety such a peptide or protein provided that the molecule is already tagged with DBCO or azide in order to react with azide or DBCO tagged lipids on the surface of the liposomes. If you are planning to use a reactive fluorophore then you need to make sure that your molecule will not have excitation or emission that can interfere with the excitation or  emission of the fluorophore that is already incorporated into the liposomes.

Download Product Insert Download Safety Datasheet (SDS)

Technical Information

PG-based Fluoroliposome®-DBCO for Tracking and Detection

Lipid CompositionConcentration (mg/ml)Concentration (mM)Molar Ratio Percentage
Total mg/ml mM100
1,2-dioleoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (sodium salt)20
1,2-dioleoyl-sn-glycero-3-phosphocholine49
Cholesterol30
1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-(dibenzocyclooctyl)1
Fluorescent DyeExcitation/Emission (nm)Molecular Structure
1,1'-Dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI) 549/565
3,3'-Dilinoleyloxacarbocyanine perchlorate (DiO)484/501
1,1'-Dioctadecyl-3,3,3',3'-tetramethylindodicarbocyanine, 4-chlorobenzenesulfonate salt (DiD)644/665
1,1'-Dioctadecyl-3,3,3',3'-tetramethylindotricarbocyanine iodide (DiR)750/780
4-(4-(Dihexadecylamino)styryl)-N-methylpyridinium iodide (DiA)
456/590
1,2-Distearoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (ammonium salt) (NBD on head group)460/535
1-Palmitoyl-2-{12-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]dodecanoyl}-sn-glycero-3-phosphocholine (NBD on fatty acid tail)460/534
1,2-Dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (ammonium salt) (Rhodamine lipid)560/583
1,2-Dioleoyl-sn-glycero-3-phosphoethanolamine-N-(5-dimethylamino-1-naphthalenesulfonyl) (ammonium salt) (Dansyl lipid)

336/513
1,2-Dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(1-pyrenesulfonyl) (ammonium salt) (Pyrene lipid)351/379
Buffer and Liposome SizeSpecification
BufferPhosphate Buffered Saline*
pH7.4
Liposome Size100 nm
* If you prefer different buffers, please specify in your order.

Technical Notes

  • If you are planning to conjugate a molecule to the surface of the liposomes then keep in mind that PG-based liposomes are highly negative in charge. It is better to use a reactive dye or molecule that is more neutral in charge rather than a dye or molecule that has a strong positive charge which can cause aggregation. 
  • Liposomes should be kept at 4 °C and NEVER be frozen.

Appearance

PG-based Fluoroliposome®-Azide formulation is a colored translucent liquid, and the color depends on the type of the fluorescent dye that is used. Usually due to the small size of liposomes no settling will occur in the bottom of the vial. The liposomes are packaged in an amber vial.

Ordering/Shipping Information

  • All liposome based formulations are shipped on blue ice at 4 °C in insulated packages using overnight shipping or international express shipping.
  • Liposomes should NEVER be frozen. Crystal ice that is formed in the lipid membrane can rupture the membrane, change the size of the liposomes and cause the encapsulated drug to leak out. Liposomes in liquid form should always be kept in the refrigerator.
  • Clients who order from outside of the United States of America are responsible for their government import taxes and customs paperwork. Encapsula NanoSciences is NOT responsible for importation fees to countries outside of the United States of America.
  • We strongly encourage the clients in Japan, Korea, Taiwan and China to order via a distributor. Tough customs clearance regulations in these countries will cause delay in custom clearance of these perishable formulations if ordered directly through us. Distributors can easily clear the packages from customs. To see the list of the distributors click here.
  • Clients ordering from universities and research institutes in Australia should keep in mind that the liposome formulations are made from synthetic material and the formulations do not require a “permit to import quarantine material”. Liposomes are NOT biological products.
  • If you would like your institute’s FedEx or DHL account to be charged for shipping then please provide the account number at the time of ordering.
  • Encapsula NanoSciences has no control over delays due to inclement weather or customs clearance delays. You will receive a FedEx or DHL tracking number once your order is confirmed. Contact FedEx or DHL in advance and make sure that the paperwork for customs is done on time. All subsequent shipping inquiries should be directed to Federal Express or DHL.

Storage and Shelf Life

Storage

PG-based Fluoroliposome®-DBCO should always be stored at in the dark at 4 °C, except when brought to room temperature for brief periods prior to animal dosing. DO NOT FREEZE. ENS is not responsible for results generated by frozen product.

Shelf Life

Fluoroliposome® products are made on daily basis. The batch that is shipped is manufactured on the same day. It is advised to use the products within 60 days of the manufacturing date.

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