dsDNA: Mass to/from Moles Convertor
This tool will convert dsDNA mass to moles of dsDNA, moles dsDNA ends, and number of DNA molecules and also moles of dsDNA to mass of dsDNA.
Formula
moles dsDNA (mol) = mass of dsDNA (g)/((length of dsDNA (bp) x 617.96 g/mol/bp) + 36.04 g/mol)
moles of dsDNA ends = moles dsDNA (mol) x 2
DNA copy number = moles of dsDNA x 6.022e23 molecules/mol
Expanded Formula
dsDNA Mass to Moles
moles dsDNA = mass of dsDNA (g)/molecular weight of dsDNA (g/mol)
molecular weight of dsDNA = (number of base pairs of dsDNA x average molecular weight of a base pair) + 36.04 g/mol
average molecular weight of a base pair = 617.96 g/mol, excluding the water molecule removed during polymerization.
The 36.04 g/mol accounts for the 2 -OH and 2 -H added back to the ends.
Bases are assumed to be unmodified.
dsDNA Mass to Moles of Ends
moles of dsDNA ends = moles linear dsDNA (mol) x 2
(see above to calculate moles dsDNA from mass)
Bases are assumed to be unmodified.
dsDNA Mass to Molecules
number of molecules dsDNA = moles of dsDNA x Avagadro's Number
Avagadro's Number = 6.022e23 molecules/mol
(see above to calculate moles dsDNA from mass)
Bases are assumed to be unmodified.
Formula
mass of dsDNA (g) = moles of dsDNA (mol) x ((length of dsDNA (bp) x 617.96 g/mol/bp) + 36.04 g/mol)
Expanded Formula
dsDNA Moles to Mass
mass of dsDNA = moles dsDNA (mol) x molecular weight of dsDNA
molecular weight of dsDNA = (number of base pairs of dsDNA x average molecular weight of a base pair) + 36.04 g/mol
average molecular weight of a base pair = 617.96 g/mol, excluding the water molecule removed during polymerization.
The 36.04 g/mol accounts for the 2 -OH and 2 -H added back to the ends.
Bases are assumed to be unmodified.
ssDNA: Mass to/from Moles Convertor
This tool will convert ssDNA mass to moles of DNA, moles of DNA ends, and number of DNA molecules and also covert moles of ssDNA to mass of ssDNA.
Formula
moles ssDNA (mol) = mass of ssDNA (g)/((length of ssDNA (nt) x 308.97 g/mol/nt) + 18.02 g/mol)
moles of ssDNA ends = moles ssDNA (mol)
DNA copy number = moles of ssDNA x 6.022e23 molecules/mol
Expanded Formula
ssDNA Mass to Moles
moles ssDNA = mass of ssDNA (g)/molecular weight of ssDNA (g/mol)
molecular weight of ssDNA = (number of deoxynucleotide monophosphates of ssDNA x average molecular weight of a deoxynucleotide monophosphate) + 18.02 g/mol
average molecular weight of a deoxynucleotide monophosphate = 308.97 g/mol, excluding the water molecule removed during polymerization.
The 18.02 g/mol accounts for the -OH and -H added back to the ends.
Bases are assumed to be unmodified.
ssDNA Mass to Moles of Ends
moles of ssDNA ends = moles linear ssDNA (mol)
(see above to calculate moles ssDNA from mass)
Bases are assumed to be unmodified.
ssDNA Mass to Molecules
ssDNA copy number = moles of ssDNA x Avagadro's Number
Avagadro's Number = 6.022e23 molecules/mol
(see above to calculate moles ssDNA from mass)
Formula
mass of ssDNA (g) = moles of ssDNA (mol) x ((length of ssDNA (nt) x 308.97 g/mol/bp) + 18.02 g/mol)
Expanded Formula
ssDNA Moles to Mass
mass of ssDNA = moles ssDNA (mol) x molecular weight of ssDNA
molecular weight of ssDNA = (number of deoxynucleotide monophosphates of ssDNA x average molecular weight of a deoxynucleotide monophosphate) + 18.02 g/mol
average molecular weight of a deoxynucleotide monophosphate = 308.97 g/mol, excluding the water molecule removed during polymerization.
The 18.02 g/mol accounts for the -OH and -H added back to the ends.
Bases are assumed to be unmodified.
ssRNA: Mass to/from Moles Convertor
This tool will convert ssRNA mass to moles of RNA, moles of RNA ends, and number of RNA molecules and also convert moles of ssRNA to mass of ssRNA.
Formula
moles ssRNA (mol) = mass of ssRNA (g)/((length of ssRNA (nt) x 321.47 g/mol) + 18.02 g/mol)
moles of ssRNA ends = moles ssRNA (mol)
RNA copy number = moles of ssRNA x 6.022e23 molecules/mol
Expanded Formula
ssRNA Mass to Moles
moles ssRNA = mass of ssRNA (g)/molecular weight of ssRNA (g/mol)
molecular weight of ssRNA = (number of ribonucleotides of ssRNA x average molecular weight of a ribonucleotide) + 18.02 g/mol
average molecular weight of a ribonucleotide = 321.47 g/mol, excluding the water molecule removed during polymerization.
The 18.02 g/mol accounts for the -OH and -H added back to the ends.
Bases are assumed to be unmodified.
ssRNA Mass to Moles of Ends
moles of ssRNA ends = moles linear ssRNA (mol)
(see above to calculate moles ssRNA from mass)
Bases are assumed to be unmodified.
ssRNA Mass to Molecules
ssRNA copy number = moles of ssRNA x Avagadro's Number
Avagadro's Number = 6.022e23 molecules/mol
(see above to calculate moles ssRNA from mass)
Formula
mass of ssRNA (g) = moles of ssRNA (mol) x ((length of ssRNA (nt) x 321.47 g/mol/nt) + 18.02 g/mol)
Expanded Formula
ssRNA Moles to Mass
mass of ssRNA = moles ssRNA (mol) x molecular weight of ssRNA
molecular weight of ssRNA = (number of nucleotides of ssRNA x average molecular weight of a nucleotide) + 18.02 g/mol
average molecular weight of a ribonucleotide = 321.47 g/mol, excluding the water molecule removed during polymerization.
The 18.02 g/mol accounts for the -OH and -H added back to the ends.
Bases are assumed to be unmodified.
