Buy Liposomes

Micron sized liposomes engineered specifically to target macrophages in different tissues and organs. The kits come in 2 different varieties: standard kits and mannosylated kits (mainly to target central nervous system). These kits are also available with various fluorescent control reagents such as DiI (ex/em: 549/565 nm), DiO (ex/em:484/501 nm), DiD (ex/em: 644/665 nm), DiA (ex/em: 456/590 nm) and DiR (ex/em: 750/780 nm) liposomes.

• Standard Macrophage Depletion Kits

  Each standard macrophage depletion kit is composed of one vial of clodronate encapsulated liposomes (Clodrosome®) and one vial of plain liposomes for control (Encapsome®) with the same lipid composition, size and buffer as Clodrosome®. Standard kits are recommended for depletion of macrophages in majority of organs and tissues except central nervous system (CNS). Fluorescent standard kits also contain a fluorescent liposome reagent (Fluoroliposome®) which is mainly used for tracking and detection. Fluorescent liposomes come in five different varieties such as DiA, DiD, DiI, DiO and DiR liposomes.

• Mannosylated Macrophage Depletion Kits

  Each mannosylated macrophage depletion kit is composed of one vial of mannosylated clodronate encapsulated liposomes (m-Clodrosome®) and one vial of plain mannosylated liposomes for control (m-Encapsome®) with the same lipid composition size and buffer as m-Clodrosome®. Mannosylated liposomes are recommended for depletion of macrophages in central nervous system (CNS). Mannosylated fluorescent kits also contain a mannosylated fluorescent liposome reagent (m-Fluoroliposome®) which is mainly used for tracking and detection. Mannosylated fluorescent liposomes come in five different varieties such as mannosylated DiA, DiD, DiI, DiO and DiR liposomes.

Liposomal doxorubicin comes in two form- PEGylated (DoxosomeTM) and non-PEGylated (DoxosomeTM-NP). Doxorubicin drug is pre-loaded into PEGylated liposomes using ammonium sulfate gradient and it is ready to be injected. Non-PEGylated liposomes comes in three vial kit. pH gradient has to be made and the drug has to be loaded into the liposomes right before use.

Surface reactive Doxorubicin liposome (Immunodox®) formulations come in various PEGylated kits and with various conjugation chemistries for conjugation to N-terminus, C-teminus, reactive sulfur or molecules containing reactive moieties such as biotin, azide, DBCO and aldehyde and folate.

• Non Surface Reactive Doxorubicin Liposomes (Doxosome™)

  Liposomal doxorubicin comes in two form- PEGylated (DoxosomeTM) and non-PEGylated (DoxosomeTM-NP).

  Doxorubicin drug is pre-loaded into PEGylated liposomes (DoxosomeTM) using ammonium sulfate gradient and it is ready to be injected. 

  Non-PEGylated liposomes (DoxosomeTM-NP) comes in three vial kit. Vial one is the liposome solution in an acidic buffer. Vial two is the basic solution that should be added to vial one in order to generate a pH gradient for loading doxorubicin drug and vial three is the doxorubicin solution that should be added to liposomes in pH gradient. Due to the pH gradient the drug will be remote loaded into the liposomes. Because of the fast leakage of the drug from liposomes made from unsaturated lipids, doxorubicin loaded liposomes should be used immediately after loading. 

• Surface Reactive Doxorubicin Liposomes (Immunodox®)

  Surface reactive Doxorubicin liposome (Immunodox®) kits are only available in PEGylated form and are used for various conjugation chemistries such as conjugation to N-terminus of antibody or available amine, C-teminus of antibody or available carboxyl,  reactive sulfur  such as Fab’ or thiolated antibodies or molecules containing reactive moieties such as biotin (for (strept)avidin bridge formation), azide and DBCO (for click chemistry), aldehyde (for reaction to hydrazide), folate (for targeting folate receptors or folate binding proteins (FBP)).

Due to the rapid hydrolysis of ATP molecules, ATP liposomes are freeze dried. These freeze dried liposomes are call proliposomes.

Proliposomes should be hydrated with deionized water right before use. ATPsome® kits are available in various formulations such as PEGylated, non-PEGylated and florescent. 

If the uptake of the liposomes by a particular cell line is known then it would wise to study the uptake of the liposomes 

• PEGylated ATP Liposomes

  There are various kits with different lipid composition and lipid charges (cationic, anionic and neutral) containing DSPE-PEG(2000) lipid which make long circulating liposomes.

• Non-PEGylated ATP Liposomes

  There are various kits with different lipid composition and lipid charges (cationic, anionic and neutral). These liposomes are not long circulating and are suitable for in vivo and in vitro studies.

There are many chemistries that can be used for conjugation of antibodies and other reactive molecules to the surface of the liposomes. Surface reactive liposomes can conjugate to N-terminus or C-terminus of antibodies. They can conjugate to free sulfur on thiolated antibody or Fab' fragment.

• Immunoliposomes (Immunosome®)

  These formulations contain a reactive lipid on the surface of the liposomes that can be used only with water soluble antibodies, proteins, peptides and ligands.

• Fluorescent Immunoliposomes (ImmunoFluor™)

  These liposomes contain a fluorescent dye for tracking. These liposomes are formulated with various types of lipophilic dyes with various excitation and emission ranges and they also contain various reactive lipids on the surface for conjugation to water soluble molecules.

• Fluorescent Liposomes - Liposomes for Pore Forming Experiments and Leakage Assay

  These liposomes are used in experiments that involve pore formation and disruption of the   membrane. They come in positive (DOTAP-based), negative (phosphatidylglycerol- and phosphatidylserine-based) and neutral charge (phosphatidylcholine-based) in order to study the impact of zeta potential on pore formation or disruption by external factors such  detergents, peptides, etc. These liposomes encapsulate fluorescent NBD tagged dextran with various sizes. Depending on the size of the pore or the level of disruption some dextran molecules can leak out of the liposomes and some dextran molecules can not and based on that information, the size of the pore can be estimated.

• Fluorescent Liposomes - Liposomes for Fusion Experiments

  These liposomes  are used in experiments that involve the fusion of two separate membranes. They come in positive (DOTAP-based), negative (phosphatidylglycerol- and phosphatidylserine-based) and neutral charge (phosphatidylcholine-based) in order to study the impact of zeta potential on membrane fusion. There are two different formulations of the liposomes that are used for the fusion experiments; one formulation co-encapsulate both NBD and rhodamine, and the other formulation contains a self-quenching concentration of octadecyl of rhodamine B.

• Fluorescent Liposomes - Liposomes for Tracking and Detection

  Fluorescent liposomes are extensively used for tracking and detection. This Fluoroliposome® catalog contains liposomes with various surface charges: positive charge (DOTAP based liposomes), negative charge (phosphatidylserine and phosphatidylglycerol based liposomes) and neutral charge (phosphatidylcholine based liposomes). These liposomes come with various lipophilic fluorophores incorporated into the lipid membrane of the liposomes with different excitation and emission ranges such as DiA (ex/em: 456/590 nm) , DiD (ex/em: 644/665 nm), DiI (ex/em: 549/565 nm) . DiO (ex/em 484/501 nm), DiR (ex/em: 750/780 nm), NBD headgroup tagged lipid (ex/em: 460/535 nm), NBD fatty acid tail tagged lipid (ex/em: 460/534), rhodamine headgroup tagged lipid (ex/em: 560/583 nm) , dansyl headgroup tagged lipid (ex/em: 336/513 nm) and pyrene headgroup tagged lipid (ex/em: 351/379 nm).

  These liposomes are also available with reactive lipids such as DBCO and azide on surface which makes them suitable for click chemistry. If you need to conjugate a specific dye with a certain property and excitation and emission range to the surface of the liposomes then you can use these surface reactive lipids for conjugation. The surface reactive liposomes also contain various types of fluorophores.

• Fluorescent Liposomes - Surface Reactive Liposomes (ImmunoFluor™)

  There are many chemistries that can be used for conjugation of antibodies and other reactive molecules to the surface of liposomes. Surface reactive liposomes can conjugate to N-terminus or C-terminus of antibodies or molecules containing reactive moieties such as biotin, azide, DBCO and aldehyde and folate. They can conjugate to free sulfurs on the thiolated antibodies or Fab' fragments. These liposomes contain a fluorescent dye for tracking. These liposomes are formulated with various types of lipophilic dyes,which have various excitation and emission ranges, and they also contain various reactive lipids on the surface for conjugation to water soluble molecules.

• Fluorescent Liposomes - Liposomes for Macrophage Uptake

  These liposomes are multilamellar and micron sized that are specifically engineered to be easily uptaken by macrophages. They are formulated using five different fluorescent dyes that cover a wide spectrum of wavelengths such as DiI (ex/em: 549/565 nm), DiO (ex/em:484/501 nm), DiD (ex/em: 644/665 nm), DiA (ex/em: 456/590 nm) and DiR (ex/em: 750/780 nm) liposomes.

These are various types of cationic liposomes that are used extensively for delivery of genetic materials such as DNA, pDNA, RNA, siRNA into the cells by in vivo and in vitro administration. In most cases the genetic materials are added externally to the liposomes in order to form a complex by electrostatic interaction.

• DOTAP based Liposomes (Genesome®-DOTAP)

  These DOTAP based cationic liposomes suitable for gene delivery (DNA/RNA delivery) are available in various formulations that are suitable for in vivo and in vitro administration. DOTAP based liposomes containing DOPE are mostly suitable for in vitro studies and DOTAP based liposomes containing cholesterol are mostly suitable for in vivo studies. These formulations are also available in fluorescent using lipids that are either tagged with NBD (ex/em: 460/535 nm) or rhodamine (ex/em: 560/583 nm) or lipophilic dyes such as DiI (549/565 nm) and DiO (484/501 nm).

• DDAB based Liposomes (Genesome®-DDAB)

  These DDAB based cationic liposomes suitable for gene delivery (DNA/RNA delivery) are available in various formulations that are suitable for in vivo and in vitro administration. These formulations are also available in fluorescent using lipids that are either tagged with NBD (ex/em: 460/535 nm) or rhodamine (ex/em: 560/583 nm).

• DOTMA based Liposomes (Genesome®-DOTMA)

  These DOTMA based cationic liposomes suitable for gene delivery (DNA/RNA delivery) are available in various formulations that are suitable for in vivo and in vitro administration. DOTMA is an analogue of DOTAP in which there is an esther bond between fatty acid and propyl backbone instead of an ester bond in DOTAP. These formulations are also available in fluorescent using lipids that are either tagged with NBD (ex/em: 460/535 nm) or rhodamine (ex/em: 560/583 nm) or lipophilic dyes such as DiI (549/565 nm) and DiO (484/501 nm).

• DC-Cholesterol based Liposomes (Genesome®-DC-Cholesterol)

  These DC-cholesterol based cationic liposomes suitable for gene delivery (DNA/RNA delivery) are available in various formulations that are suitable for in vivo and in vitro administration. DC-Cholesterol is a cationic analogue of cholesterol. DC cholesterol by itself doesn't form liposomes and it should be added to a matrix lipid.  These formulations are also available in fluorescent using lipids that are either tagged with NBD (ex/em: 460/535 nm) or rhodamine (ex/em: 560/583 nm).

• GL-67 based Liposomes (Genesome®-GL-67)

  These GL-67 based cationic liposomes suitable for gene delivery (DNA/RNA delivery) are available in various formulations that are suitable for in vivo and in vitro administration. GL-67 is a cationic analogue of cholesterol. GL-67 by itself doesn't form liposomes and it should be added to a matrix lipid.  These formulations are also available in fluorescent using lipids that are either tagged with NBD (ex/em: 460/535 nm) or rhodamine (ex/em: 560/583 nm).

Plain liposomes are extensively used as artificial cell models and for studying the interaction of various types of molecules such as peptides and proteins with the surface of the lipid membranes. Plain liposomes are also used as control formulations for many different types of drug encapsulated liposome formulations. Charged liposomes are also used in many different types of blood complement studies. Plain liposomes are available in various anionic, cationic and neutral surface charges. Plain liposomes can be unilamellar or mulitamellar.

• Liposomes made from Natural Lipid Extracts

  Cellsome®-Natural extract catalog is composed of many liposomes made from phosphatidylcholine (PC) or phosphatidylserine (PS) lipids that are made from natural lipids that are extracted from egg, soy, E.coli, yeast and also tissues and organs such as brain, heart and liver.

• Liposomes used as Exosome Models

  Plain liposomes composed of L-alpha-phosphatidylcholine and cholesterol which are sized to 100 nm are extensively used as exosome model control.

• Liposomes made from Diether Lipids

  Diether lipids do not go through hydrolysis due to having an ether bond instead of an acyl bond and therefore to due that they are a suitable candidates for experiments that needs to be performed at a higher temperature for an extended period of time. In addition to that phospholipase A2 can not hydrolyze ether lipid liposomes.

• Liposomes made from Saturated Lipids

  Cellsome®-Saturated PC catalog is composed of many products made from saturated lipids with different fatty acid length from 12:0-24:0 with and without cholesterol. Saturated lipids have a high liquid to gel phase transition temperature.

• Cationic Liposomes (DOTAP-based)

  DOTAP is a positively charged molecule and the products in Cellsome®-DOTAP-based catalog use DOPC as a matrix lipid and various amounts of cationic DOTAP lipid from 0.5% to 50% with various zeta potentials. If you are looking for DOTAP based liposomes for gene delivery then you need to see the Genesome® liposome catalog.

• Liposomes made from PEGylated Lipids

  PEGylated liposomes or Stealth liposomes or long circulating liposomes have been the subject of many studies for the past three decades. Cellsome®-PEGylated catalog products contain anionic, cationic and neutral charged liposomes containing 5 mol percent DSPE-PEG(2000) lipid without any encapsulated molecules which are suitable for many different types of experiments.

• Liposomes made from Unsaturated Lipids

  Cellsome®-Unsaturated PC catalog is composed of many products made from unsaturated lipids with different fatty acid length and degree of saturation from 14:1-20:4. Unsaturated lipids have a negative liquid to gel phase transition temperature.

• Anionic Liposomes

  Phosphatidylserine (PS) and phosphatidylglycerol (PG) are two well known anionic molecules that form liposomes with negative zeta potential. The zeta potential of the liposomes vary depending on the molar percentage of the negatively charged lipids in the liposome formulation. Anionic Cellsome®-Phosphatidylserine (PS) and Cellsome®-Phosphatidylglycerol (PG) catalogs contain the products made from 0.5 up to 100 percent DOPS and DOPG with various amount of negative zeta potentials.

• Liposomes made from Cardiolipin Lipids

  Cardiolipid is a very unique lipid with various biochemical functions. It is a dimer of phosphatidylglycerol and phosphatidic acid containing four acyl chains and two phosphate headgroup. Cardiolipin is a negatively charged lipid. Cellsome® made from cardiolipin lipid catalog containing many different type of saturated and unsaturated cardiolipin based liposomes made from 0.5 up to 100 percent of cardiolipin.

Lyophosome™ product catalog is composed of a large selection of freeze dried liposomes with various types of lipids and wide range of zeta potentials and different properties. Lyophosome™ products should be used by scientists who understand liposome formulation and have the proper equipment to check the size, separate non-encapsulated drugs and do the proper assays. Freeze dried liposomes cannot be used blindly.

• Lyophilized Liposomes made from Natural Lipid Extracts

  Lyophosome™-Natural extract catalog is composed of many freeze-dried liposomes made from phosphatidylcholine (PC) or phosphatidylserine (PS) lipids that are made from natural lipids that are extracted from egg, soy, E. coli, yeast and also tissues and organs such as brain, heart and liver.

• Lyophilized Liposomes made from Diether Lipids

  These products are freeze-dried liposomes made from diether lipids. Diether lipids do not go through hydrolysis due to having an ether bond instead of an acyl bond and therefore to due that they are a suitable candidates for experiments that needs to be performed at a higher temperature for an extended period of time. In addition to that phospholipase A2 can not hydrolyze ether lipid liposomes.

• Lyophilized Liposomes made from Saturated Lipids

  Lyophosome™-Saturated PC catalog is composed of many freeze-dried liposomes made from saturated lipids with different fatty acid length from 12:0-24:0 with and without cholesterol. Saturated lipids have a high liquid to gel phase transition temperature.

• Lyophilized Cationic Liposomes (DOTAP-based)

  These products are freeze-dried DOTAP-based liposomes. DOTAP is a positively charged molecule and the products in Lyophosome™-DOTAP-based catalog use DOPC as a matrix lipid and various amounts of cationic DOTAP lipid from 0.5% to 50% with various zeta potentials. If you are looking for DOTAP based liposomes for gene delivery then you need to see the Genesome® liposome catalog.

• Lyophilized Anionic Liposomes

  Phosphatidylserine (PS) and phosphatidylglycerol (PG) are two well-known anionic molecules that form the liposomes with negative zeta potential. The zeta potential of the liposomes varies, depending on the molar percentage of the negatively charged lipids in the liposome formulation. Anionic Lyophosome™-Phosphatidylserine (PS) and Lyophosome™-Phosphatidylglycerol (PG) catalogs contain freeze-dried liposomes made from 0.5 up to 100 percent DOPS and DOPG with various amount of negative zeta potentials.

• Lyophilized Liposomes made from PEGylated Lipids

  PEGylated liposomes or Stealth liposomes or long circulating liposomes have been the subject of many studies for the past three decades. Lyophosome™-PEGylated catalog products contain anionic, cationic and neutral freeze-dried liposomes containing 5 mol percent DSPE-PEG(2000) lipid without any encapsulated molecules which are suitable for many different types of experiments.

• Lyophilized Liposomes made from Unsaturated Lipids

  Lyophosome™-Unsaturated PC catalog is composed of many freeze-dried liposomes made from unsaturated lipids with different fatty acid length and degree of saturation from 14:1-20:4. Unsaturated lipids have a negative liquid to gel phase transition temperature.

• Liposomal Vitamin A
• Liposomal Vitamin C
• Liposomal Vitamin E
• Liposomal CBD
• Liposomal CoQ10
• Liposomal Resveratrol
• Liposomal Aloe Vera
• Liposomal Vitamin ACE
• Liposomes containig Anti-wrinkle peptide