PEGylated DOTA Liposomes for Remote Loading Radioactive Divalent Cations

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2-ml DTAG-201 $950.00
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Description

DOTA encapsulated liposomes are used for remote loading of radioactive divalent ions such as radioactive copper into the liposomes. Cationic ions will not pass through the membrane if there is no pH gradient. Divalent ion will cross the liposome membrane only when there is a flux of proton across of the lipid membrane. Due to the difference in the pH inside and outside of the liposomes, there will be a constant flux of proton from inside of the liposomes to outside of the liposomes. Due to this flux, divalent cations move in the opposite direction from outside to inside of liposomes. Liposomes are thermodynamically stable structures and not very permeable to divalent cations after they are formed. If there is no pH gradient divalent cations can not move from outside to inside of the liposomes and they will remain outside and won’t cross the membrane. The driving force for remote loading in proton gradient.

The radioactive divalent copper is externally added to the liposomes. The divalent ion passes the membrane using pH gradient method and chelates DOTA.

Download Product Insert Download Safety Datasheet (SDS)

Formulation Information

PEGylated DOTA Liposomes for Remote Loading Radioactive Divalent Cations

Lipid CompositionConcentration (mg/ml)Concentration (mM)Molar Ratio Percentage
Total 15 mg/ml mM100
1,2-distearoyl-sn-glycero-3-phosphocholine55
Cholesterol40
DSPE-PEG(2000)5
Buffer, Liposome Size and Encapsulated DOTASpecification
BufferHEPES Buffered Saline
pH4
Liposome size100 nm
1,4,7,10-Tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA)10 mM
Vial 2Specification
Sodium Carbonate166 mM
pH11.4

Technical Notes

  • Liposomes should be kept at 4 °C and NEVER be frozen.

Appearance

DOTA liposome is a white translucent liquid made of nano size unilamellar liposomes. Usually due to the small size of liposomes no settling will occur in the bottom of the vial. The liposomes are packaged in an amber vial.

Ordering/Shipping Information

  • All liposome based formulations are shipped on blue ice at 4 °C in insulated packages using overnight shipping or international express shipping.
  • Liposomes should NEVER be frozen. Crystal ice that is formed in the lipid membrane can rupture the membrane and change the size of the liposomes. Liposomes in liquid form should always be kept in the refrigerator.
  • Clients who order from outside of the United States of America are responsible for their government import taxes and customs paperwork. Encapsula NanoSciences is NOT responsible for importation fees to countries outside of the United States of America.
  • We strongly encourage the clients in Japan, Korea, Taiwan and China to order via a distributor. Tough customs clearance regulations in these countries will cause delay in custom clearance of these perishable formulations if ordered directly through us. Distributors can easily clear the packages from customs. To see the list of the distributors click here.
  • Clients ordering from universities and research institutes in Australia should keep in mind that the liposome formulations are made from synthetic material and the formulations do not require a “permit to import quarantine material”. Liposomes are NOT biological products.
  • If you would like your institute’s FedEx or DHL account to be charged for shipping then please provide the account number at the time of ordering.
  • Encapsula NanoSciences has no control over delays due to inclement weather or customs clearance delays. You will receive a FedEx or DHL tracking number once your order is confirmed. Contact FedEx or DHL in advance and make sure that the paperwork for customs is done on time. All subsequent shipping inquiries should be directed to Federal Express or DHL.

Storage and Shelf Life

Storage

DOTA liposome should always be stored at in the dark at 4 °C, except when brought to room temperature for brief periods prior to animal dosing. DO NOT FREEZE. If the suspension is frozen, the encapsulated drug can be released from the liposomes thus limiting its effectiveness. In addition, the size of the liposomes will also change upon freezing and thawing.

Shelf Life

DOTA liposomes are made on daily basis. The batch that is shipped is manufactured on the same day. It is advised to use the products within 4 months of the manufacturing date.

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