Lipid Composition for Fluorescent Cationic PEGylated ATP Liposomes (ATPsome®-PEG) – DOTAP-based

Lipid Composition for FADG-201	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
1,2-dioleoyl-3-trimethylammonium-propane (chloride salt)	0.975	0.68	65
Cholesterol	0.435	0.17	29
1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethyleneglycol)- 2000] (ammonium salt)	0.075	0.21	5
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (ammonium salt) (NBD PE)	0.015	0.01	1
Total	1.500 µmol/vial	1.07 mg/vial	100

Lipid Composition for FADG-202	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
1,2-dioleoyl-3-trimethylammonium-propane (chloride salt)	0.900	0.63	60
1,2-dioleoyl-sn-glycero-3-phosphocholine	0.075	0.06	5
Cholesterol	0.435	0.17	29
1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethyleneglycol)- 2000] (ammonium salt)	0.075	0.21	5
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (ammonium salt) (NBD PE)	0.015	0.01	1
Total	1.500 µmol/vial	1.08 mg/vial	100

Lipid Composition for FADG-203	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
1,2-dioleoyl-3-trimethylammonium-propane (chloride salt)	0.750	0.52	50
1,2-dioleoyl-sn-glycero-3-phosphocholine	0.225	0.18	15
Cholesterol	0.435	0.17	29
1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethyleneglycol)- 2000] (ammonium salt)	0.075	0.21	5
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (ammonium salt) (NBD PE)	0.015	0.01	1
Total	1.500 µmol/vial	1.09 mg/vial	100

Lipid Composition for FADG-204	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
1,2-dioleoyl-3-trimethylammonium-propane (chloride salt)	0.600	0.42	40
1,2-dioleoyl-sn-glycero-3-phosphocholine	0.375	0.29	25
Cholesterol	0.435	0.17	29
1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethyleneglycol)- 2000] (ammonium salt)	0.075	0.21	5
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (ammonium salt) (NBD PE)	0.015	0.01	1
Total	1.500 µmol/vial	1.10 mg/vial	100

Lipid Composition for FADG-205	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
1,2-dioleoyl-3-trimethylammonium-propane (chloride salt)	0.450	0.31	30
1,2-dioleoyl-sn-glycero-3-phosphocholine	0.525	0.41	35
Cholesterol	0.435	0.17	29
1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethyleneglycol)- 2000] (ammonium salt)	0.075	0.21	5
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (ammonium salt) (NBD PE)	0.015	0.01	1
Total	1.500 µmol/vial	1.11 mg/vial	100

Lipid Composition for FADG-206	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
1,2-dioleoyl-3-trimethylammonium-propane (chloride salt)	0.300	0.21	20
1,2-dioleoyl-sn-glycero-3-phosphocholine	0.675	0.53	45
Cholesterol	0.435	0.17	29
1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethyleneglycol)- 2000] (ammonium salt)	0.075	0.21	5
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (ammonium salt) (NBD PE)	0.015	0.01	1
Total	1.500 µmol/vial	1.13 mg/vial	100

Lipid Composition for FADG-207	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
1,2-dioleoyl-3-trimethylammonium-propane (chloride salt)	0.150	0.10	10
1,2-dioleoyl-sn-glycero-3-phosphocholine	0.825	0.65	55
Cholesterol	0.435	0.17	29
1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethyleneglycol)- 2000] (ammonium salt)	0.075	0.21	5
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (ammonium salt) (NBD PE)	0.015	0.01	1
Total	1.500 µmol/vial	1.14 mg/vial	100

Lipid Composition for FADG-208	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
1,2-dioleoyl-3-trimethylammonium-propane (chloride salt)	0.075	0.05	5
1,2-dioleoyl-sn-glycero-3-phosphocholine	0.900	0.71	60
Cholesterol	0.435	0.17	29
1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethyleneglycol)- 2000] (ammonium salt)	0.075	0.21	5
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (ammonium salt) (NBD PE)	0.015	0.01	1
Total	1.500 µmol/vial	1.16 mg/vial	100

Lipid Composition for FADG-209	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
1,2-dioleoyl-3-trimethylammonium-propane (chloride salt)	0.0375	0.03	2.5
1,2-dioleoyl-sn-glycero-3-phosphocholine	0.9375	0.74	62.5
Cholesterol	0.4350	0.17	29
1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethyleneglycol)- 2000] (ammonium salt)	0.0750	0.21	5
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (ammonium salt) (NBD PE)	0.0150	0.01	1
Total	1.5000 µmol/vial	1.16 mg/vial	100

Lipid Composition for FADG-210	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
1,2-dioleoyl-3-trimethylammonium-propane (chloride salt)	0.015	0.01	1
1,2-dioleoyl-sn-glycero-3-phosphocholine	0.960	0.75	64
Cholesterol	0.435	0.17	29
1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethyleneglycol)- 2000] (ammonium salt)	0.075	0.21	5
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (ammonium salt) (NBD PE)	0.015	0.01	1
Total	1.500 µmol/vial	1.15 mg/vial	100

Lipid Composition for FADG-211	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
1,2-dioleoyl-3-trimethylammonium-propane (chloride salt)	0.975	0.68	65
Cholesterol	0.435	0.17	29
1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethyleneglycol)- 2000] (ammonium salt)	0.075	0.21	5
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (ammonium salt) (Rhod PE)	0.015	0.02	1
Total	1.500 µmol/vial	1.08 mg/vial	100

Lipid Composition for FADG-212	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
1,2-dioleoyl-3-trimethylammonium-propane (chloride salt)	0.900	0.63	60
1,2-dioleoyl-sn-glycero-3-phosphocholine	0.075	0.06	5
Cholesterol	0.435	0.17	29
1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethyleneglycol)- 2000] (ammonium salt)	0.075	0.21	5
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (ammonium salt) (Rhod PE)	0.015	0.02	1
Total	1.500 µmol/vial	1.09 mg/vial	100

Lipid Composition for FADG-213	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
1,2-dioleoyl-3-trimethylammonium-propane (chloride salt)	0.750	0.52	50
1,2-dioleoyl-sn-glycero-3-phosphocholine	0.225	0.18	15
Cholesterol	0.435	0.17	29
1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethyleneglycol)- 2000] (ammonium salt)	0.075	0.21	5
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (ammonium salt) (Rhod PE)	0.015	0.02	1
Total	1.500 µmol/vial	1.10 mg/vial	100

Lipid Composition for FADG-214	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
1,2-dioleoyl-3-trimethylammonium-propane (chloride salt)	0.600	0.42	40
1,2-dioleoyl-sn-glycero-3-phosphocholine	0.375	0.29	25
Cholesterol	0.435	0.17	29
1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethyleneglycol)- 2000] (ammonium salt)	0.075	0.21	5
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (ammonium salt) (Rhod PE)	0.015	0.02	1
Total	1.500 µmol/vial	1.11 mg/vial	100

Lipid Composition for FADG-215	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
1,2-dioleoyl-3-trimethylammonium-propane (chloride salt)	0.450	0.31	30
1,2-dioleoyl-sn-glycero-3-phosphocholine	0.525	0.41	35
Cholesterol	0.435	0.17	29
1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethyleneglycol)- 2000] (ammonium salt)	0.075	0.21	5
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (ammonium salt) (Rhod PE)	0.015	0.02	1
Total	1.500 µmol/vial	1.12 mg/vial	100

Lipid Composition for FADG-216	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
1,2-dioleoyl-3-trimethylammonium-propane (chloride salt)	0.300	0.21	20
1,2-dioleoyl-sn-glycero-3-phosphocholine	0.675	0.53	45
Cholesterol	0.435	0.17	29
1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethyleneglycol)- 2000] (ammonium salt)	0.075	0.21	5
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (ammonium salt) (Rhod PE)	0.015	0.02	1
Total	1.500 µmol/vial	1.14 mg/vial	100

Lipid Composition for FADG-217	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
1,2-dioleoyl-3-trimethylammonium-propane (chloride salt)	0.150	0.10	10
1,2-dioleoyl-sn-glycero-3-phosphocholine	0.825	0.65	55
Cholesterol	0.435	0.17	29
1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethyleneglycol)- 2000] (ammonium salt)	0.075	0.21	5
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (ammonium salt) (Rhod PE)	0.015	0.02	1
Total	1.500 µmol/vial	1.15 mg/vial	100

Lipid Composition for FADG-218	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
1,2-dioleoyl-3-trimethylammonium-propane (chloride salt)	0.075	0.05	5
1,2-dioleoyl-sn-glycero-3-phosphocholine	0.900	0.71	60
Cholesterol	0.435	0.17	29
1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethyleneglycol)- 2000] (ammonium salt)	0.075	0.21	5
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (ammonium salt) (Rhod PE)	0.015	0.02	1
Total	1.500 µmol/vial	1.16 mg/vial	100

Lipid Composition for FADG-219	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
1,2-dioleoyl-3-trimethylammonium-propane (chloride salt)	0.0375	0.03	2.5
1,2-dioleoyl-sn-glycero-3-phosphocholine	0.9375	0.74	62.5
Cholesterol	0.4350	0.17	29
1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethyleneglycol)- 2000] (ammonium salt)	0.0750	0.21	5
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (ammonium salt) (Rhod PE)	0.0150	0.02	1
Total	1.5000 µmol/vial	1.17 mg/vial	100

Lipid Composition for FADG-220	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
1,2-dioleoyl-3-trimethylammonium-propane (chloride salt)	0.015	0.01	1
1,2-dioleoyl-sn-glycero-3-phosphocholine	0.960	0.75	64
Cholesterol	0.435	0.17	29
1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethyleneglycol)- 2000] (ammonium salt)	0.075	0.21	5
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (ammonium salt) (Rhod PE)	0.015	0.02	1
Total	1.500 µmol/vial	1.16 mg/vial	100