Lipid Composition for Cationic PEGylated ATP Liposomes (ATPsome®-PEG) – DOTAP-based

Lipid Composition for ADPG-201	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
1,2-dioleoyl-3-trimethylammonium-propane (chloride salt)	0.975	0.68	65
Cholesterol	0.450	0.17	30
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (ammonium salt)	0.075	0.21	5
Total	1.500 µmol/vial	1.06 mg/vial	100

Lipid Composition for ADPG-202	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
1,2-dioleoyl-3-trimethylammonium-propane (chloride salt)	0.900	0.63	60
1,2-dioleoyl-sn-glycero-3-phosphocholine	0.075	0.06	5
Cholesterol	0.450	0.17	30
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (ammonium salt)	0.075	0.21	5
Total	1.500 µmol/vial	1.07 mg/vial	100

Lipid Composition for ADPG-203	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
1,2-dioleoyl-3-trimethylammonium-propane (chloride salt)	0.750	0.52	50
1,2-dioleoyl-sn-glycero-3-phosphocholine	0.225	0.18	15
Cholesterol	0.450	0.17	30
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (ammonium salt)	0.075	0.21	5
Total	1.500 µmol/vial	1.08 mg/vial	100

Lipid Composition for ADPG-204	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
1,2-dioleoyl-3-trimethylammonium-propane (chloride salt)	0.600	0.42	40
1,2-dioleoyl-sn-glycero-3-phosphocholine	0.375	0.29	25
Cholesterol	0.450	0.17	30
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (ammonium salt)	0.075	0.21	5
Total	1.500 µmol/vial	1.09 mg/vial	100

Lipid Composition for ADPG-205	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
1,2-dioleoyl-3-trimethylammonium-propane (chloride salt)	0.450	0.31	30
1,2-dioleoyl-sn-glycero-3-phosphocholine	0.525	0.41	35
Cholesterol	0.450	0.17	30
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (ammonium salt)	0.075	0.21	5
Total	1.500 µmol/vial	1.10 mg/vial	100

Lipid Composition for ADPG-206	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
1,2-dioleoyl-3-trimethylammonium-propane (chloride salt)	0.300	0.21	20
1,2-dioleoyl-sn-glycero-3-phosphocholine	0.675	0.53	45
Cholesterol	0.450	0.17	30
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (ammonium salt)	0.075	0.21	5
Total	1.500 µmol/vial	1.12 mg/vial	100

Lipid Composition for ADPG-207	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
1,2-dioleoyl-3-trimethylammonium-propane (chloride salt)	0.150	0.10	10
1,2-dioleoyl-sn-glycero-3-phosphocholine	0.825	0.65	55
Cholesterol	0.450	0.17	30
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (ammonium salt)	0.075	0.21	5
Total	µmol/vial	1.13 mg/vial	100

Lipid Composition for ADPG-208	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
1,2-dioleoyl-3-trimethylammonium-propane (chloride salt)	0.075	0.05	5
1,2-dioleoyl-sn-glycero-3-phosphocholine	0.900	0.71	60
Cholesterol	0.450	0.17	30
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (ammonium salt)	0.075	0.21	5
Total	1.500 µmol/vial	1.14 mg/vial	100

Lipid Composition for ADPG-209	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
1,2-dioleoyl-3-trimethylammonium-propane (chloride salt)	0.0375	0.03	2.5
1,2-dioleoyl-sn-glycero-3-phosphocholine	0.9375	0.74	62.5
Cholesterol	0.4500	0.17	30
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (ammonium salt)	0.0750	0.21	5
Total	1.5000 µmol/vial	1.15 mg/vial	100

Lipid Composition for ADPG-210	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
1,2-dioleoyl-3-trimethylammonium-propane (chloride salt)	0.015	0.01	1
1,2-dioleoyl-sn-glycero-3-phosphocholine	0.960	0.75	64
Cholesterol	0.450	0.17	30
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (ammonium salt)	0.075	0.21	5
Total	1.500 µmol/vial	1.14 mg/vial	100