Lipid Composition for Fluorescent Phosphatidylglycerol (PG)-based ATP Liposomes (ATPsome®)

Lipid Composition for FAG-201	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
1,2-dioleoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (sodium salt)	1.050	0.84	70
Cholesterol	0.435	0.17	29
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (ammonium salt) (NBD PE)	0.015	0.01	1
Total	1.500 µmol/vial	1.02 mg/vial	100

Lipid Composition for FAG-202	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
1,2-dioleoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (sodium salt)	0.900	0.72	60
1,2-dioleoyl-sn-glycero-3-phosphocholine	0.150	0.12	10
Cholesterol	0.435	0.17	29
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (ammonium salt) (NBD PE)	0.015	0.01	1
Total	1.500 µmol/vial	1.02 mg/vial	100

Lipid Composition for FAG-203	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
1,2-dioleoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (sodium salt)	0.750	0.60	50
1,2-dioleoyl-sn-glycero-3-phosphocholine	0.300	0.24	20
Cholesterol	0.435	0.17	29
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (ammonium salt) (NBD PE)	0.015	0.01	1
Total	1.500 µmol/vial	1.02 mg/vial	100

Lipid Composition for FAG-204	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
1,2-dioleoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (sodium salt)	0.600	0.48	40
1,2-dioleoyl-sn-glycero-3-phosphocholine	0.450	0.35	30
Cholesterol	0.435	0.17	29
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (ammonium salt) (NBD PE)	0.015	0.01	1
Total	1.500 µmol/vial	1.01 mg/vial	100

Lipid Composition for FAG-205	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
1,2-dioleoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (sodium salt)	0.450	0.36	30
1,2-dioleoyl-sn-glycero-3-phosphocholine	0.600	0.47	40
Cholesterol	0.435	0.17	29
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (ammonium salt) (NBD PE)	0.015	0.01	1
Total	1.500 µmol/vial	1.01 mg/vial	100

Lipid Composition for FAG-206	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
1,2-dioleoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (sodium salt)	0.300	0.24	20
1,2-dioleoyl-sn-glycero-3-phosphocholine	0.750	0.59	50
Cholesterol	0.435	0.17	29
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (ammonium salt) (NBD PE)	0.015	0.01	1
Total	1.500 µmol/vial	1.01 mg/vial	100

Lipid Composition for FAG-207	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
1,2-dioleoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (sodium salt)	0.150	0.12	10
1,2-dioleoyl-sn-glycero-3-phosphocholine	0.900	0.71	60
Cholesterol	0.435	0.17	29
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (ammonium salt) (NBD PE)	0.015	0.01	1
Total	1.500 µmol/vial	1.01 mg/vial	100

Lipid Composition for FAG-208	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
1,2-dioleoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (sodium salt)	0.075	0.06	5
1,2-dioleoyl-sn-glycero-3-phosphocholine	0.975	0.77	65
Cholesterol	0.435	0.17	29
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (ammonium salt) (NBD PE)	0.015	0.01	1
Total	1.500 µmol/vial	1.01 mg/vial	100

Lipid Composition for FAG-209	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
1,2-dioleoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (sodium salt)	0.038	0.03	2.5
1,2-dioleoyl-sn-glycero-3-phosphocholine	1.013	0.80	67.5
Cholesterol	0.435	0.17	29
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (ammonium salt) (NBD PE)	0.015	0.01	1
Total	1.500 µmol/vial	1.01 mg/vial	100

Lipid Composition for FAG-210	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
1,2-dioleoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (sodium salt)	0.015	0.01	1
1,2-dioleoyl-sn-glycero-3-phosphocholine	1.035	0.81	69
Cholesterol	0.435	0.17	29
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (ammonium salt) (NBD PE)	0.015	0.01	1
Total	1.500 µmol/vial	1.00 mg/vial	100

Lipid Composition for FAG-211	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
1,2-dioleoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (sodium salt)	0.008	0.01	0.5
1,2-dioleoyl-sn-glycero-3-phosphocholine	1.043	0.82	69.5
Cholesterol	0.435	0.17	29
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (ammonium salt) (NBD PE)	0.015	0.01	1
Total	1.500 µmol/vial	1.01 mg/vial	100

Lipid Composition for FAG-212	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
1,2-dioleoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (sodium salt)	1.050	0.84	70
Cholesterol	0.435	0.17	29
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (ammonium salt) (Rhod PE)	0.015	0.02	1
Total	1.500 µmol/vial	1.03 mg/vial	100

Lipid Composition for FAG-213	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
1,2-dioleoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (sodium salt)	0.900	0.72	60
1,2-dioleoyl-sn-glycero-3-phosphocholine	0.150	0.12	10
Cholesterol	0.435	0.17	29
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (ammonium salt) (Rhod PE)	0.015	0.02	1
Total	1.500 µmol/vial	1.03 mg/vial	100

Lipid Composition for FAG-214	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
1,2-dioleoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (sodium salt)	0.750	0.60	50
1,2-dioleoyl-sn-glycero-3-phosphocholine	0.300	0.24	20
Cholesterol	0.435	0.17	29
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (ammonium salt) (Rhod PE)	0.015	0.02	1
Total	1.500 µmol/vial	1.03 mg/vial	100

Lipid Composition for FAG-215	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
1,2-dioleoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (sodium salt)	0.600	0.48	40
1,2-dioleoyl-sn-glycero-3-phosphocholine	0.450	0.35	30
Cholesterol	0.435	0.17	29
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (ammonium salt) (Rhod PE)	0.015	0.02	1
Total	1.500 µmol/vial	2.02 mg/vial	100

Lipid Composition for FAG-216	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
1,2-dioleoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (sodium salt)	0.450	0.36	30
1,2-dioleoyl-sn-glycero-3-phosphocholine	0.600	0.47	40
Cholesterol	0.435	0.17	29
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (ammonium salt) (Rhod PE)	0.015	0.02	1
Total	1.500 µmol/vial	1.02 mg/vial	100

Lipid Composition for FAG-217	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
1,2-dioleoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (sodium salt)	0.300	0.24	20
1,2-dioleoyl-sn-glycero-3-phosphocholine	0.750	0.59	50
Cholesterol	0.435	0.17	29
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (ammonium salt) (Rhod PE)	0.015	0.02	1
Total	1.500 µmol/vial	1.02 mg/vial	100

Lipid Composition for FAG-218	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
1,2-dioleoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (sodium salt)	0.150	0.12	10
1,2-dioleoyl-sn-glycero-3-phosphocholine	0.900	0.71	60
Cholesterol	0.435	0.17	29
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (ammonium salt) (Rhod PE)	0.015	0.02	1
Total	1.500 µmol/vial	1.02 mg/vial	100

Lipid Composition for FAG-219	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
1,2-dioleoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (sodium salt)	0.075	0.06	5
1,2-dioleoyl-sn-glycero-3-phosphocholine	0.975	0.77	65
Cholesterol	0.435	0.17	29
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (ammonium salt) (Rhod PE)	0.015	0.02	1
Total	1.500 µmol/vial	1.02 mg/vial	100

Lipid Composition for FAG-220	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
1,2-dioleoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (sodium salt)	0.038	0.03	2.5
1,2-dioleoyl-sn-glycero-3-phosphocholine	1.013	0.80	67.5
Cholesterol	0.435	0.17	29
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (ammonium salt) (Rhod PE)	0.015	0.02	1
Total	1.500 µmol/vial	1.02 mg/vial	100

Lipid Composition for FAG-221	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
1,2-dioleoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (sodium salt)	0.015	0.01	1
1,2-dioleoyl-sn-glycero-3-phosphocholine	1.035	0.81	69
Cholesterol	0.435	0.17	29
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (ammonium salt) (Rhod PE)	0.015	0.02	1
Total	1.500 µmol/vial	1.01 mg/vial	100

Lipid Composition for FAG-222	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
1,2-dioleoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (sodium salt)	0.008	0.01	0.5
1,2-dioleoyl-sn-glycero-3-phosphocholine	1.043	0.82	69.5
Cholesterol	0.435	0.17	29
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (ammonium salt) (Rhod PE)	0.015	0.02	1
Total	1.500 µmol/vial	1.02 mg/vial	100