Lipid Composition for Fluorescent Phosphatidylserine (PS)-based ATP Liposomes (ATPsome®)

Lipid Composition for FAS-201	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
L-alpha-phosphatidylserine	1.485	1.20	99
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (ammonium salt) (NBD PE)	0.015	0.01	1
Total	1.500 µmol/vial	1.21 mg/vial	100

Lipid Composition for FAS-202	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
L-alpha-phosphatidylserine	1.350	1.09	90
L-alpha-phosphatidylcholine	0.135	0.10	9
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (ammonium salt) (NBD PE)	0.015	0.01	1
Total	1.500 µmol/vial	1.20 mg/vial	100

Lipid Composition for FAS-203	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
L-alpha-phosphatidylserine	1.200	0.97	80
L-alpha-phosphatidylcholine	0.285	0.22	19
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (ammonium salt) (NBD PE)	0.015	0.01	1
Total	1.500 µmol/vial	1.20 mg/vial	100

Lipid Composition for FAS-204	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
L-alpha-phosphatidylserine	1.050	0.85	70
L-alpha-phosphatidylcholine	0.435	0.34	29
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (ammonium salt) (NBD PE)	0.015	0.01	1
Total	1.500 µmol/vial	1.20 mg/vial	100

Lipid Composition for FAS-205	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
L-alpha-phosphatidylserine	0.900	0.73	60
L-alpha-phosphatidylcholine	0.585	0.45	39
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (ammonium salt) (NBD PE)	0.015	0.01	1
Total	1.500 µmol/vial	1.19 mg/vial	100

Lipid Composition for FAS-206	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
L-alpha-phosphatidylserine	0.750	0.60	50
L-alpha-phosphatidylcholine	0.735	0.57	49
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (ammonium salt) (NBD PE)	0.015	0.01	1
Total	1.500 µmol/vial	1.18 mg/vial	100

Lipid Composition for FAS-207	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
L-alpha-phosphatidylserine	0.600	0.48	40
L-alpha-phosphatidylcholine	0.885	0.68	59
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (ammonium salt) (NBD PE)	0.015	0.01	1
Total	1.500 µmol/vial	1.17 mg/vial	100

Lipid Composition for FAS-208	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
L-alpha-phosphatidylserine	0.450	0.36	30
L-alpha-phosphatidylcholine	1.035	0.80	69
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (ammonium salt) (NBD PE)	0.015	0.01	1
Total	1.500 µmol/vial	1.17 mg/vial	100

Lipid Composition for FAS-209	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
L-alpha-phosphatidylserine	0.300	0.24	20
L-alpha-phosphatidylcholine	1.185	0.91	79
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (ammonium salt) (NBD PE)	0.015	0.01	1
Total	1.500 µmol/vial	1.16 mg/vial	100

Lipid Composition for FAS-210	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
L-alpha-phosphatidylserine	0.150	0.12	10
L-alpha-phosphatidylcholine	1.335	1.03	89
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (ammonium salt) (NBD PE)	0.015	0.01	1
Total	1.500 µmol/vial	1.16 mg/vial	100

Lipid Composition for FAS-211	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
L-alpha-phosphatidylserine	1.485	1.20	99
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (ammonium salt) (Rhod PE)	0.015	0.01	1
Total	1.500 µmol/vial	1.21 mg/vial	100

Lipid Composition for FAS-212	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
L-alpha-phosphatidylserine	1.350	1.09	90
L-alpha-phosphatidylcholine	0.135	0.10	9
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (ammonium salt) (Rhod PE)	0.015	0.02	1
Total	1.500 µmol/vial	1.21 mg/vial	100

Lipid Composition for FAS-213	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
L-alpha-phosphatidylserine	1.200	0.97	80
L-alpha-phosphatidylcholine	0.285	0.22	19
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (ammonium salt) (Rhod PE)	0.015	0.02	1
Total	1.500 µmol/vial	1.21 mg/vial	100

Lipid Composition for FAS-214	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
L-alpha-phosphatidylserine	1.050	0.85	70
L-alpha-phosphatidylcholine	0.435	0.34	29
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (ammonium salt) (Rhod PE)	0.015	0.02	1
Total	1.500 µmol/vial	1.21 mg/vial	100

Lipid Composition for FAS-215	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
L-alpha-phosphatidylserine	0.900	0.73	60
L-alpha-phosphatidylcholine	0.585	0.45	39
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (ammonium salt) (Rhod PE)	0.015	0.02	1
Total	1.500 µmol/vial	1.20 mg/vial	100

Lipid Composition for FAS-216	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
L-alpha-phosphatidylserine	0.750	0.60	50
L-alpha-phosphatidylcholine	0.735	0.57	49
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (ammonium salt) (Rhod PE)	0.015	0.02	1
Total	1.500 µmol/vial	1.19 mg/vial	100

Lipid Composition for FAS-217	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
L-alpha-phosphatidylserine	0.600	0.48	40
L-alpha-phosphatidylcholine	0.885	0.68	59
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (ammonium salt) (Rhod PE)	0.015	0.02	1
Total	1.500 µmol/vial	1.18 mg/vial	100

Lipid Composition for FAS-218	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
L-alpha-phosphatidylserine	0.450	0.36	30
L-alpha-phosphatidylcholine	1.035	0.80	69
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (ammonium salt) (Rhod PE)	0.015	0.02	1
Total	1.500 µmol/vial	1.18 mg/vial	100

Lipid Composition for FAS-219	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
L-alpha-phosphatidylserine	0.300	0.24	20
L-alpha-phosphatidylcholine	1.185	0.91	79
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (ammonium salt) (Rhod PE)	0.015	0.02	1
Total	1.500 µmol/vial	1.17 mg/vial	100

Lipid Composition for FAS-220	Amount (µmol/vial)	Amount (mg/vial)	Molar Ratio Percentage
L-alpha-phosphatidylserine	0.150	0.12	10
L-alpha-phosphatidylcholine	1.335	1.03	89
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (ammonium salt) (Rhod PE)	0.015	0.02	1
Total	1.500 µmol/vial	1.17 mg/vial	100